Me yasa ake amfani da Polyacrylamide don gina jiki electrophoresis

A fagen kimiyyar zamani, furotin electrophoresis yana tsaye a matsayin dabarar ginshiƙi don tantancewa da siffanta sunadaran.A zuciyar wannan hanya ta ta'allaka nePolyacrylamide, wani abu mai mahimmanci wanda ke aiki a matsayin kashin baya na gel matrices da aka yi amfani da shi a cikin tsarin gel electrophoresis.Abubuwan musamman na Polyacrylamide sun sa ya zama kayan aiki mai mahimmanci ga masu bincike da masana kimiyya waɗanda ke neman buɗe rikitattun sunadarai da mu'amalarsu.

Polyacrylamide, wanda aka fi sani da PAM, polymer roba ce da aka yi daga acrylamide monomers.Ƙwararrensa na ban mamaki ana danganta shi da ikonsa na samar da dogayen sarƙoƙi, wanda ya haifar da wani abu mai kama da gel wanda zai iya ɗaukar nau'i-nau'i daban-daban na kwayoyin.Wannan kadarar ta sa polyacrylamide ya zama kyakkyawan ɗan takara don ƙirƙirar matrices mara kyau da aka yi amfani da su a cikin furotin electrophoresis.

Protein electrophoresis wata dabara ce da ke raba furotin bisa la'akari da cajin su da girmansu.Ta hanyar ƙaddamar da samfurin furotin zuwa filin lantarki a cikin matrix polyacrylamide gel matrix, sunadaran suna yin ƙaura ta gel a cikin nau'i daban-daban, wanda ya haifar da nau'i daban-daban waɗanda za'a iya tantancewa da ƙididdige su.Wannan rabuwa yana ba da haske mai mahimmanci game da tsarkin furotin, ƙaddarar nauyin kwayoyin halitta, da kasancewar isoforms.

Matsayin Polyacrylamide a cikin Protein Electrophoresis

Zaɓin polyacrylamide don furotin electrophoresis ya samo asali ne a cikin yanayin da ake iya daidaita shi.Masana kimiyya na iya daidaita ƙaddamar da matrix gel don ɗaukar sunadaran masu girma dabam.Maɗaukaki mafi girma yana haifar da matrices masu ƙarfi da suka dace don magance ƙananan sunadaran, yayin da ake amfani da ƙananan ƙididdiga don manyan sunadaran.Wannan daidaitawa yana tabbatar da cewa masu bincike zasu iya daidaita gwaje-gwajen su don cimma mafi kyawun rabuwa da bincike.

Polyacrylamide as aFlocculant

Polyacrylamide's mai amfani ya wuce fiye da rawar da yake takawa a cikin gel electrophoresis.Hakanan yana samun aikace-aikace azaman flocculant a masana'antu daban-daban, kamar sarrafa ruwa da sarrafa ruwan sha.A matsayin flocculant, polyacrylamide yana taimakawa wajen tara ɓangarorin da aka dakatar a cikin ruwaye, suna sauƙaƙe cire su.Wannan sifa tana ba da haske game da iyakoki daban-daban na fili da kuma tasiri mai fa'ida akan kimiyya da masana'antu.

Ci gaba a cikin Electrophoresis na tushen Polyacrylamide

'Yan shekarun nan sun shaida ci gaba da ci gaba a cikin fasahar electrophoresis na tushen polyacrylamide.PAGE na asali, SDS-PAGE, da gel electrophoresis mai nau'i-nau'i biyu kawai 'yan misalan yadda daidaitawar polyacrylamide ya ba da damar haɓaka hanyoyin na musamman don nazarin tsarin furotin, gyare-gyaren fassarar bayan fassaro, da hulɗa.Waɗannan fasahohin suna da kima a cikin bincike na proteomics da ƙoƙarin gano magunguna.

A cikin yanayin nazarin furotin, polyacrylamide yana fitowa a matsayin abokin gaba, yana ba masu bincike damar shiga cikin duniyar sunadarai.Matsayinsa na tushen tushen gel matrices a cikin tsarin electrophoresis ba za a iya wuce gona da iri ba.Daga hanyoyin warware cututtukan zuwa haɓaka sabbin hanyoyin warkewa, electrophoresis na tushen polyacrylamide yana ci gaba da haifar da ci gaban kimiyya.Yayin da fasaha ke ci gaba, wannan abin al'ajabi na roba zai iya tasowa, yana ƙara wadatar fahimtarmu game da sunadaran da ayyukansu masu yawa.