Me yasa ake amfani da Polyacrylamide don electrophoresis na furotin

A fannin kimiyyar zamani, electrophoresis na furotin yana matsayin wata babbar dabara ta nazari da kuma siffanta sunadaran. A zuciyar wannan hanya ita cePolyacrylamide, wani sinadari mai amfani wanda ke aiki a matsayin tushen matrices na gel da ake amfani da su a tsarin electrophoresis na gel. Abubuwan da Polyacrylamide ke da su na musamman sun sanya shi kayan aiki mai mahimmanci ga masu bincike da masana kimiyya waɗanda ke neman warware sarkakiyar sunadaran da hulɗarsu.

Polyacrylamide, wanda aka fi sani da PAM, wani sinadari ne na roba da aka yi daga monomers na acrylamide. An danganta shi da sauƙin amfani da shi saboda ikonsa na samar da dogayen sarƙoƙi, wanda ke haifar da wani abu mai kama da gel wanda zai iya ɗaukar nau'ikan ƙwayoyin halitta daban-daban. Wannan sinadari yana sanya polyacrylamide ya zama zaɓi mafi dacewa don ƙirƙirar matrices masu ramuka waɗanda ake amfani da su wajen samar da furotin electrophoresis.

Tsarin lantarki na furotin wata dabara ce da ke raba sunadarai bisa ga cajinsu da girmansu. Ta hanyar sanya samfurin furotin zuwa filin lantarki a cikin matrix na gel na polyacrylamide, sunadarai suna ƙaura ta cikin gel ɗin a cikin sauye-sauye daban-daban, wanda ke haifar da madaidaitan madaukai waɗanda za a iya bincika su da kuma ƙididdige su. Wannan rabuwar tana ba da fahimta mai mahimmanci game da tsarkin furotin, tantance nauyin ƙwayoyin halitta, da kuma kasancewar isoforms.

Matsayin Polyacrylamide a cikin Protein Electrophoresis

Zaɓar polyacrylamide don furotin electrophoresis ya samo asali ne daga yanayinsa mai sauƙin gyarawa. Masana kimiyya za su iya daidaita yawan gel matrix don ɗaukar furotin masu girma dabam-dabam. Mafi yawan yawan yana haifar da matrices masu tsauri waɗanda suka dace da warware ƙananan furotin, yayin da ake amfani da ƙananan yawan don manyan furotin. Wannan daidaitawa yana tabbatar da cewa masu bincike za su iya daidaita gwaje-gwajensu don cimma mafi kyawun rabuwa da bincike.

Polyacrylamide a matsayin maganiFlocculant

Amfanin Polyacrylamide ya wuce matsayinsa a cikin electrophoresis na gel. Hakanan yana samun aikace-aikace a matsayin flocculant a cikin masana'antu daban-daban, kamar maganin ruwa da sarrafa ruwan shara. A matsayin flocculant, polyacrylamide yana taimakawa wajen tattara barbashi da aka dakatar a cikin ruwa, yana sauƙaƙa cire su. Wannan halayyar tana nuna iyawar mahaɗan daban-daban da tasirin da ke tattare da su ga kimiyya da masana'antu.

Ci gaba a cikin Electrophoresis na Polyacrylamide

Shekarun baya-bayan nan sun shaida ci gaba da ci gaba a cikin dabarun electrophoresis na tushen polyacrylamide. Native PAGE, SDS-PAGE, da electrophoresis na gel mai girma biyu kaɗan ne kawai na yadda daidaitawar polyacrylamide ta ba da damar haɓaka hanyoyi na musamman don nazarin tsarin furotin, gyare-gyare bayan fassara, da hulɗa. Waɗannan dabarun suna da matuƙar amfani a cikin binciken proteomics da ƙoƙarin gano magunguna.

A fannin nazarin furotin, polyacrylamide ya fito a matsayin abokiyar aiki mai ƙarfi, wanda ke ba masu bincike damar zurfafa bincike kan duniyar sunadarai masu rikitarwa. Ba za a iya misalta rawar da yake takawa a matsayin tushen matrices na gel a cikin tsarin electrophoresis ba. Daga hanyoyin warware cututtuka zuwa haɓaka sabbin hanyoyin magani, electrophoresis na tushen polyacrylamide yana ci gaba da tsara ci gaban kimiyya. Yayin da fasaha ke ci gaba, wannan abin al'ajabi na roba zai iya bunƙasa, yana ƙara wa fahimtarmu game da sunadarai da ayyukansu iri-iri.